Potentiation of antifungal effect of amphotericin B by squalene, an intermediate for sterol biosynthesis.
- 1 January 1982
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 35 (2) , 230-234
- https://doi.org/10.7164/antibiotics.35.230
Abstract
The antifungal effect of a polyene antibiotic amphotericin B (AMB) was almost completely abrogated by exogenous addition of 0.1 .mu.g/ml ergosterol in the medium. The cytocidal effect of AMB on Saccharomyces cerevisiae was synergistically enhanced when cultured for > 4 h with squalene, an obligatory intermediate molecule for sterol formation. No significant increase in cellular level of ergosterol content in the yeast cells fed with squalene could be found.This publication has 7 references indexed in Scilit:
- Classification of polyene antibiotics according to their synergistic effect in combination with bleomycin A2 or fusidic acidAntimicrobial Agents and Chemotherapy, 1980
- Effect of fatty acids on action of polyene antibioticsAntimicrobial Agents and Chemotherapy, 1980
- Isolation and characterization of amphotericin B-resistant cell lines in Chinese hamster cellsCell, 1978
- Animal cell mutants defective in sterol metabolism: A specific selection procedure and partial characterization of defectsProceedings of the National Academy of Sciences, 1977
- MECHANISM OF PROTECTION BY STEROLS AGAINST POLYENE ANTIBIOTICSJournal of Bacteriology, 1960
- A SIMPLE METHOD FOR THE ISOLATION AND PURIFICATION OF TOTAL LIPIDES FROM ANIMAL TISSUESJournal of Biological Chemistry, 1957
- The Utilization of Squalene in the Biosynthesis of CholesterolJournal of Biological Chemistry, 1953