Kinetic Study of β-Cyclodextrin-Dye System by High-Pressure Temperature-Jump Method1
- 1 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 96 (1) , 69-72
- https://doi.org/10.1093/oxfordjournals.jbchem.a134830
Abstract
The pressure dependence of the formation and dissociation rate constants of the inclusion reaction between β-cyclodextrin and phenolphthalein was investigated with a newly constructed high-pressure temperature-jump apparatus. The jump height of temperature was 2.8°C within 1 μs under pressure up to 2,000 kg/cm 2 . The reaction was analyzed as a simple reversible bimolecular reaction. The activation volume was 1.78±0.85cm 3 /mol for the forward reaction and 10.65±1.4Ocm 3 /mol for the backward reaction. The values of activation volumes suggest that desolvation from the phenolphthalein molecule and release of hydrating water molecules from the β-cyclodextrin cavity occur at the transition state.Keywords
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