Purification and Characterization of Flagella from Roseburia cecicola, an Obligately Anaerobic Bacterium
- 1 August 1985
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 131 (8) , 2075-2078
- https://doi.org/10.1099/00221287-131-8-2075
Abstract
Flagella from R. cecicola, an obligately anaerobic bacterium originally isolated from murine cecal mucosa, were purified by mechanical shearing followed by differential centrifugation. Purity of the flagellar preparation was determined by polyacrylamide gel electrophoresis, EM and chemical analysis. The flagella were composed of a single protein subunit (flagellin) with an estimated MW of 42,000. The amino acid composition of the flagellin was similar to that of some facultatively anaerobic and aerobic bacteria.This publication has 2 references indexed in Scilit:
- Roseburia cecicola gen. nov., sp. nov., a Motile, Obligately Anaerobic Bacterium from a Mouse CecumInternational Journal of Systematic and Evolutionary Microbiology, 1983
- A new and improved microassay to determine 2-keto-3-deoxyoctonate in lipopolysaccharide of gram-negative bacteriaAnalytical Biochemistry, 1978