Growth of Prymnesium parvum in the Dark; Note on Ichthyotoxin Formation*

Abstract
SYNOPSIS. For the first time the chrysomonad Prymnesium parvum was grown in the dark; glycerol was necessary and could not be replaced by any other metabolite tested. The dark‐grown cultures, as compared to light‐grown ones, atteined an almost 2‐fold optical density per cell number, caused possibly by a partial pigment‐bleaching in the light or a higher pigment formation in the dark. Ichthyotoxic activity of Prymnesium cultures was greater for the dark‐grown cultures in which no photoinactivation of this toxin occurs. Therefore light is not required for synthesis of this toxin.