THE BIOSYNTHESIS OF COPROPORPHYRINOGEN, MAGNESIUM PROTOPORPHYRIN MONOMETHYL ESTER AND BACTERIOCHLOROPHYLL BY RHODOPSEUDOMONAS CAPSULATA

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Abstract
Suspensions of a strain of R. capsulata, incubated in the light in a mixture containing glycine and succinate, synthesized high concentrations of bacteriochlorophyll and 2 probable intermediates in its biosynthesis: coproporphyrinogen-III and magnesium-protoporphyrin-monomethyl-ester. More bacteriochlorophyll and much less coproporphyrinogen were produced with increasing concentrations of Fe. The production of magnesium-protoporphyrin-monomethyl-ester was greatest at an intermediate Fe concentration. Less bacteriochlorophyll and more coproporphyrinogen were produced with increasing concentrations of methionine. The production of magnesium-protoporphyrin-monomethyl-ester was greatest at an intermediate methionine concentration. The synthesis of magnesium-protoporphyrin-monomethyl-ester by cultures or suspensions was increased 10- to 20-fold by adding Tween-80. Coproporphyrinogen synthesized by R. capsulata was identical with the product of chemical reduction of coproporphyrin-III in all properties examined. A compound with an absorption peak in acid solution at 500 m[mu], probably the dipyrrylmethene tetrapyrrole, was intermediate in the chemical oxidation of coproporphyrinogen to coproporphyrin.

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