COLONY STIMULATING ACTIVITY IN NORMAL HUMAN-SERUM TESTED AGAINST HUMAN-BONE MARROW

  • 1 January 1980
    • journal article
    • research article
    • Vol. 8  (10) , 1232-1240
Abstract
Normal human sera (230) were tested for colony stimulating factor (CSF) under carefully controlled conditions using human bone marrow and the semi-solid agar-gel technique. CSF can be detected in most sera when incorporated into underlayers to remove potential inhibitors. The effect of storage temperature on serum CSF was determined. Storage at 4.degree.; -15.degree. and -66.degree. for up to 80 days resulted in a mild decrease in CSF levels. After 240 days of storage CSF values fell to 50% of that found before storage. Repeated freeze-thawing of serum did not decrease CSF levels when done in Pyrex glassware. These studies will serve as a baseline for studying human serum CSA in hematopoietic disorders.