Relation of Seededness and Ringing to Gibberellin-like Activity in Berries of Vitis vinifera

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Abstract
Extracts of flowers, berries, or both, of 5 varieties of grape (Vitis vinifera) were analyzed for gibberellin-like activity. Extracting was done with 3 organic solvents, chloroform, ethyl acetate, and n-butanol, at low (2.5), neutral, and basic (8.0) pH values. For the chromatographic separation of gibberellins a developing solvent of isopropanol, ammonium hydroxide, and water (10:1:1 v/v) was used. Dwarf peas grown under red light were used for the bioassay. Gibberellin-like activity was found in all varieties of grapes tested, and at least in certain instances, in all fractions tested. In the 2 varieties having both seedless and seeded forms activity was present in both types. There was more gibberellin-like activity in seeded than seedless Tokay. Ringing had a tremendous influence on gibberellin content of the berries of seedless varieties. In "Black Corinth" ringing greatly decreased the content of neutral gibberellin-like activity, but increased the activity in the acidic ethyl acetate and acidic n-butanol fractions.