Abstract
Ejaculated bull spermatozoa were exposed to various concentrations of ouabain to ascertain the effect on membrane potential, intracellular concentrations of Na and K and motility. Membrane potential, measured by electrophysiological methods, decreased. Intracellular K decreased and intracellular Na increased. Progressive motility decreased. The motility change produced by ouabain was identified as a decrease both in beat frequency and in wave amplitude.

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