Quantitative Aspects of Exchangeable Calcium in Spores of Bacillus megaterium

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Abstract
Rode , L. J. (The University of Texas, Austin), and J. W. Foster . Quantitative aspects of exchangeable calcium in spores of Bacillus megaterium . J. Bacteriol. 91: 1589–1593. 1966.—More than 90% of the calcium in Ca 45 -labeled native spores was released from the cells during germination. Some 95% of the spore calcium was not exchangeable when ungerminated native spores were titrated to p H 4 with HNO 3 . Ca, Mg, Na, Si, and Fe were displaced from the spores by H + . The adsorption of Ca 45 by H-spores and its subsequent release were studied under a variety of conditions. The isolated “coat fraction” of spores adsorbed substantial amounts of Ca 45 . Release of the adsorbed Ca was achieved with various reagents.