Rate of Transacylation between 2′ and 3′-O-L-PhenyIalanyIadenosine

Publisher Website
Google Scholar
Abstract
The 270-MHz proton NMR spectra of 2′-O-L-phenylalanyladenosine and 3′-O-L-phenylalanyladenosine in deuterated phosphate buffer were analyzed. The transacylation between these two isomers was studied by saturation transfer experiments on H1′ proton resonances and the transacylation rate was directly determined to be 0.76 s−1 at pD 6.9 and 25°C. This transacylation rate is appreciably slower than the rate of polypeptide chain elongation, suggesting the presence of enzymatic activity for the transacylation of aminoacyl-tRNA in protein biosynthesis.

This publication has 2 references indexed in Scilit: