Endothelial Cell Gene Expression of a Neutrophil Chemotactic Factor by TNF-α, LPS, and IL-1β
- 17 March 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 243 (4897) , 1467-1469
- https://doi.org/10.1126/science.2648570
Abstract
Human endothelial cells produced a neutrophil chemotactic factor (NCF) upon stimulation with tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), or lipopolysaccharide (LPS). The expression of endothelial cell-derived NCF messenger RNA and biological activity was both time- and concentration-dependent. Maximal NCF mRNA expression occurred at 10 and at 2 nanograms per milliliter for TNF and IL-1 beta, respectively; mRNA expression was first observed 1 hour after stimulation and was maintained for at least 24 hours. In situ hybridization analysis showed that NCF mRNA peaked in treated cells by 24 hours, whereas unstimulated cells were negative. These studies demonstrated that endothelial cells may participate in neutrophil-mediated inflammation by synthesizing a chemotactic factor in response to specific monokines and LPS.Keywords
This publication has 16 references indexed in Scilit:
- Molecular cloning of a human monocyte-derived neutrophil chemotactic factor (MDNCF) and the induction of MDNCF mRNA by interleukin 1 and tumor necrosis factor.The Journal of Experimental Medicine, 1988
- Tumor necrosis factor-mediated release of platelet-derived growth factor from cultured endothelial cells.The Journal of Experimental Medicine, 1987
- Cachectin/TNF as well as interleukin-1 induces prostacyclin synthesis in cultured vascular endothelial cellsBiochemical and Biophysical Research Communications, 1986
- Recombinant tumor necrosis factor induces procoagulant activity in cultured human vascular endothelium: characterization and comparison with the actions of interleukin 1.Proceedings of the National Academy of Sciences, 1986
- Tumor necrosis factor/cachectin interacts with endothelial cell receptors to induce release of interleukin 1.The Journal of Experimental Medicine, 1986
- Stimulation of the adherence of neutrophils to umbilical vein endothelium by human recombinant tumor necrosis factor.Proceedings of the National Academy of Sciences, 1985
- Epidermal keratin gene expressed in embryos of Xenopus laevis.Proceedings of the National Academy of Sciences, 1985
- Molecular Cloning of the Complementary DNA for Human Tumor Necrosis FactorScience, 1985
- Rapid quantitation of neutrophil chemotaxis: Use of a polyvinylpyrrolidone-free polycarbonate membrane in a multiwell assemblyJournal of Immunological Methods, 1980
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979