Lipid Extraction from Channel Catfish Muscle: Comparison of Solvent Systems

Publisher Website
Google Scholar
Abstract
During extraction of lipids from catfish minced muscle using 9 solvent systems, phospholipid and triacylglycerol recoveries were similar when tissue was mixed on a vortex (under nitrogen) with about 20 vol of chloroform:methanol (2:1), hexane:isopropanol (3:2), chloroform :isopropanol (7:11), dichloromethane:methanol (2:l) or chloro‐form:methanol:water (2:2:1). Only a few minutes were required to extract a tissue sample by these methods as compared to a few hours for a Soxhlet extraction (petroleum ether) or for extraction by a dry column procedure (dichloromethane:methanol, 9:l). The time available for interaction of the muscle tissue with the dichlorome‐thane:methanol (9:l) system was critical in extraction of lipids, particularly phospholipids.