Is the C‐terminal flanking peptide of rat cholecystokinin double sulphated?
Open Access
- 3 February 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 196 (1) , 5-8
- https://doi.org/10.1016/0014-5793(86)80203-4
Abstract
A specific radioimmunoassay was developed to the predicted nine amino acid C‐terminal flanking peptide of cholecystokinin (peptide serine serine, PSS). In aqueous extracts of rat brain, PSS was undetectable unless the extracts were first treated with arylsulphatase, which also resulted in desulphation of cholecystokinin. The reverse‐phase HPLC analysis of partially desulphated extracts showed the presence of two peaks intermediate to the naturally occurring and the completely desulphated forms. It is therefore proposed that the CCK‐flanking peptide PSS has both tyrosine residues sulphated.Keywords
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