CRYSTALLINE DESOXYRIBONUCLEASE
Open Access
- 20 March 1950
- journal article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 33 (4) , 363-377
- https://doi.org/10.1085/jgp.33.4.363
Abstract
A study was made of the enzymatic properties of crystalline desoxyribonuclease. The general effect of the crystalline enzyme on its specific substrate, thymus nucleic acid, was found to be essentially the same as described by previous workers for the digestive action of crude preparations of the enzyme. The digestive action consists mainly in splitting thymus nucleic acid into fragments approaching the size of tetranucleotides. The digested nucleic acid is diffusible through collodion or cellophane membranes and is non-precipitable with strong acid, alcohol, or proteins. The digestion of thymus nucleic acid by desoxyribonuclease is accompanied by the liberation of one atom equivalent of free acid per four atoms of nucleic acid phosphorus.Keywords
This publication has 3 references indexed in Scilit:
- CRYSTALLINE DESOXYRIBONUCLEASEThe Journal of general physiology, 1950
- ON THE SIGNIFICANCE OF MAGNESIUM ION FOR DESOXYRIBONUCLEASE ACTIVITYJournal of Biological Chemistry, 1949
- EVIDENCE OF THE EXISTENCE OF A CORE IN DESOXYRIBONUCLEIC ACIDSJournal of Biological Chemistry, 1949