VITAMIN C AS A COENZYME: THE HYDROLYSIS OF MUSTARD OIL GLUCOSIDES

1 December 1961

journal article
research article
Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences

Vol. 47 (12) , 1875-1880
https://doi.org/10.1073/pnas.47.12.1875

Abstract

Two enzymes, myrosin and ascorbate-activated glucosinolase were identified in mustard seed (Sinapsis alba). Both of these hydrolyze the glucoside, sinigrin, found in mustard flour. The enzyme containing the ascorbate ion resulted in a increase in the cleavage rate of sinigrin. Acidic as well as basic catalysis was exerted on the substrate by the coenzymatic reaction with ascorbate, and this was accompanied by reduction and inhibition of the enzyme.

Keywords

CARBOHYDRASES/CHEMISTRY
COENZYMES/CHEMISTRY
MUSTARD/CHEMISTRY
VITAMIN C/CHEMISTRY

This publication has 2 references indexed in Scilit:

A Mammalian Thioglycosidase
Science, 1959
A thioglucosidase in fungi
Archives of Biochemistry and Biophysics, 1958