Simultaneous recordings of glucose dependent electrical activity and ATP‐regulated K+‐currents in isolated mouse pancreatic β‐cells

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Abstract
Membrane potential and membrane currents were recorded from single mouse pancreatic β‐cells using the perforated patch whole‐cell recording technique at 30 °C. Single β‐cells maintained in primary tissue culture exhibited glucose‐dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1±0.9 nS) was determined by ATP‐regulated K+ (KATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at −70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of KATP channel activity do not underlie the slow waves.