Translational fusion with a secretory enzyme as an indicator
- 1 July 1987
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 169 (7) , 3082-3087
- https://doi.org/10.1128/jb.169.7.3082-3087.1987
Abstract
A novel type of translational fusion system has been developed by using a secretory protein, staphylococcal beta-lactamase, as an indicator. The beta-lactamase structural gene was modified to provide N-terminal extensions of 13 and 162 amino acids, and in both cases, the fusion protein was processed and the mature active enzyme was secreted; thus, the expression of a particular upstream gene can be analyzed by monitoring the beta-lactamase activity.This publication has 48 references indexed in Scilit:
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