EFFECT OF 1,25(OH)2D3 AND 24,25(OH)2D3 ON EXPERIMENTAL RICKETS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 26  (3) , 571-580
Abstract
The vitamin D3 metabolite 1,25(OH)2D3 alone may not be able to reverse defective bone mineralization, while a combination with another metabolite, 24,25(OH)2D3 might be necessary to display the known effect of vitamin D on bone. Growing rachitic rats were treated with 25 ng vitamin D3/d (day), 10 ng 1,25(OH)2D3/d, 10 ng 24,25(OH)2D3/d, 5 ng 1,25(OH)2D3/d and 5 ng 24,25(OH)2D3/d, in combination and 10 ng 1,25(OH)2D3/d and 10 ng 24,25(OH)2D3/d in combination. After 10 days of treatment the epiphyseal plate width of femur and the distance of tetracycline fluorescence bands were measured microscopically on undecalcified sections of the bone. The Ca content of femur epiphysis was measured by neutron activation analysis. Neither parameter showed a greater effect of the combination of 1,25(OH)2D3 and 24,25(OH)2D3 than of 1,25(OH)2D3 alone.