Isolation and Bacterial Expression of a Murine Alpha Leukocyte Interferon Gene
- 1 January 1984
- journal article
- research article
- Published by Mary Ann Liebert Inc in Journal of Interferon Research
- Vol. 4 (4) , 635-643
- https://doi.org/10.1089/jir.1984.4.635
Abstract
A murine alpha interferon gene (MuIFN-αA) was isolated from a murine genomic DNA library in phage lambda. The gene was cloned and its sequence determined. It was expressed in Escherichia coli under the control of the trp promoter/operator which resulted in antiviral activity on mouse L-cells. MuIFN-αA exhibits 60% homology to HuIFN-αA at the amino acid level.Keywords
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