COMPARISON OF RADIOIMMUNOASSAY FOR INTERNAL PROTEIN OF BOVINE LEUKEMIA-VIRUS WITH NEUTRALIZATION TEST EMPLOYING VSV-BLV PSEUDOTYPE
- 1 January 1980
- journal article
- research article
- Vol. 27 (5) , 517-523
Abstract
Two methods for the detection of antibodies to the bovine leukemia virus (BLV) in infected animals were compared for their suitability for the early diagnosis of bovine leukemia, pseudotype neutralization test (PNT) employing vesicular stomatitis virus-bovine leukemia virus pseudotypes (VSV-BLV) and radioimmunoassay test (RIA) for major internal viral protein p24 of the BLV. The comparison was made using > 300 sera from cows of the herds with high incidence of bovine leukemia. In infected animals the presence of antibodies against virus envelope glycoprotein detected by PNT and antibodies against major structural viral protein p24 detected by RIA were always found coincidentally. Both methods were highly comparable and suitable for early detection of bovine leukemia virus infected animals.This publication has 2 references indexed in Scilit:
- PSEUDOTYPE PARTICLES OF VESICULAR STOMATITIS-VIRUS WITH SURFACE-ANTIGENS OF BOVINE LEUKEMIA-VIRUS VSV (BLV) - SENSITIVE PROBE FOR DETECTING ANTIBODIES IN SERA OF SPONTANEOUSLY INFECTED CATTLE1978
- Biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virusJournal of Virology, 1977