Initiation of general recombination catalyzed in vitro by the recA protein of Escherichia coli.
- 1 June 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (6) , 2615-2619
- https://doi.org/10.1073/pnas.76.6.2615
Abstract
Homogeneous recA protein catalyzes the hybridization of single-stranded DNA to homologous regions in duplex DNA. The products are D-loops, which are formed with equal efficiency in linear and supercoiled molecules. This assimilation reaction can be separated into two partial reactions. In the first, recA protein binds to duplex DNA and produces a reA protein-DNA complex. The binding shows a sigmoidal dependence on recA protein concentration, requires ATP, GTP or the gamma-thio analog of ATP, and Mg2+, but does not require hydrolysis of the nucleoside triphosphate. In the second reaction, single-stranded regions of the recA protein-ATP-duplex DNA intermediate hybridize with free complementary single strands to produce D-loop structures. This reaction is coupled to ATP hydrolysis and is analogous to the renaturation of single-stranded DNA catalyzed by the recA protein [Weinstrock, G.M., McEntee, K. & Lehman, I.R. (1979) Proc. Natl. Acad. Sci. USA 76, 126-130]. Hydrolysis of ATP appears to be required in these reactions for dissociation of recA protein from the DNA.Keywords
This publication has 12 references indexed in Scilit:
- Purified Escherichia coli recA protein catalyzes homologous pairing of superhelical DNA and single-stranded fragments.Proceedings of the National Academy of Sciences, 1979
- ATP-dependent renaturation of DNA catalyzed by the recA protein of Escherichia coli.Proceedings of the National Academy of Sciences, 1979
- Energy coupling in DNA gyrase and the mechanism of action of novobiocinProceedings of the National Academy of Sciences, 1978
- Genetic Recombination: Strand Transfer and Mismatch RepairAnnual Review of Biochemistry, 1978
- Protein X is the product of the recA gene of Escherichia coli.Proceedings of the National Academy of Sciences, 1977
- Uptake of homologous single-stranded fragments by superhelical DNAJournal of Molecular Biology, 1977
- Uptake of homologous single-stranded fragments by superhelical DNAJournal of Molecular Biology, 1977
- Recombination promoted by superhelical DNA and the recA gene of Escherichia coli.Proceedings of the National Academy of Sciences, 1976
- RECOMBINATION DEFICIENT MUTANTS OF E. COLI AND OTHER BACTERIAAnnual Review of Genetics, 1973
- Synthesis and maturation of phage P22 DNAJournal of Molecular Biology, 1968