Light- and Electron Microscope Studies on the Anterior Pituitary (Übergangsteil of STENDELL) of the Carp (Cyprinus carpio LINNÉ)

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Abstract
The main part of the adenohypophysis that is the Übergangsteil, pars principalis or meso-adenohypophysis of the carp was investigated through the light- and electron microscopes. This part of the carp pituitary consists of glandular cells of three types, acidophiles, basophiles and chromophobes.Both of the acidophiles and basophiles contain a great number of secretory granules which are proved to be produced at the GOLGI apparatus with the eleccron microscope. Two types of granules were seen in both the acidophiles and basophiles. One type of the granules of acidophiles is regularly round but another is irregular in shape, either elongate, dumbbell-shaped or tadpole-like. Some cells of the acidophile group contain exclusively round granules with few exceptions, while others contain the granules mostly irregular in shape. Sometimes cells with mixed round and irregular granules can be observed. These two types of granules in the acidophile could not be distinguished from each other by light microscopy.In the basophiles, there also exist two types of granules, one is small and about the same in size as acidophile but another may reach the size as large as a half of the nucleus. The latter has been repeatedly noticed by the early investigators and often called the globules. They are acidophilic in contrast to the basophilia of small granules when stained with azan method after fixation with ZENKER-formol. The globules are positively stained with both of the lipid stainings (Sudan black B, Sudan III and BAKER's acid hematein) and the protein detection (mercuric bromphenol blue). They are also positive to periodic acid-SCHIFF reaction, aldehyde fuchsin and chrome-alumhematoxylin stainings. Therefore, it is possible to conjecture that the globules may be glucolipoprotein.Electron microscopic observations revealed a large globule which contains many small secretory granules and vesicles with their limiting membranes. This finding may suggest a possibility that small granules and vesicles may gather into a large body and that coalescence of small granules may bring forth the large globules. Membraneus elements of lipoprotein structure intermingled with conglutinating granules may be responsible to the phospholipid moiety of the large globules which stain with fat-staining dyes especially with BAKER'S acid hematein.

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