Dibutyryl cyclic GMP and hyperventilation promote rat lung phospholipid release

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Abstract
Ventilation of rats at high inspiratory pressures raises lung tissue content of cyclic[c]GMP. Hyperventilation in rabbits augments release of phospholipid into lavage fluid. To determine whether cGMP, in the absence of hyperventilation, can increase lung phospholipid release, Sprague-Dawley rats were injected with [14C]palmitate and after 1.5 h were anesthetized and ventilated for 20 min. Three groups were ventilated at peak inspiratory pressures (PIP) of 10 cmH2O, while saline, dibutyryl adenosine 3''5''-cyclic monophosphate (DBcAMP) or dibutyryl cGMP (DBcGMP) was infused into the pulmonary artery. In a 4th group, saline was infused into the pulmonary artery, but ventilation was performed with PIP of 25 cmH2O. Lung tissue and lavage fluid were analyzed for phospholipid (PL) content and for incorporation of [14C]palmitate into lavage and tissue PL fractions. Ventilation at increases pressure and infusion of DBcGMP were associated with increases in release of labeled PL into lavage fraction. The increase in lavage PL release associated with hyperventilation is apparently at least in part mediated by cGMP.