Purification of Prealbumin from Human and Canine Serum Using a Two-Step Affinity Chromatographic Procedure

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Abstract
A 2- step method is described by which human and canine prealbumin were isolated from serum in good yield and high purity. The 1st step involves binding and release of prealbumin by a thiol-disulfide interchange reaction with thiol-Sepharose 4B. The 2nd step consists of reversible binding of prealbumin to retinol-binding protein covalently linked to Sepharose 4B. The canine prealbumin was immunochemically identifed as an .alpha./2-globulin. Like human prealbumin it is a tetramer with some 10% smaller subunits than human prealbumin. Both proteins are devoid of carbohydrate and both bind thyroxine.

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