Biotin synthase mechanism: on the origin of sulphur
Open Access
- 27 November 1998
- journal article
- Published by Wiley in FEBS Letters
- Vol. 440 (1-2) , 226-230
- https://doi.org/10.1016/s0014-5793(98)01464-1
Abstract
Biotin synthase catalyses the last step of the biosynthesis of biotin in microorganisms and plants. The active protein isolated from Bacillus sphaericus and Escherichia coli contains an iron‐sulphur (FeS) cluster. The native enzymes were depleted of their iron and inorganic sulphide and the resulting apoenzymes were chemically reconstituted with FeCl3 and Na2[34S] to give labelled (Fe34S) enzymes. These enzymes were functional and when assayed in vitro produced labelled biotin containing about 65% of 34S. These data strongly support the hypothesis that the sulphur of biotin is derived from the (FeS) centre of the enzyme.Keywords
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