Binding of Glucosyltransferase and Glucan Synthesis by Streptococcus mutans and Other Bacteria

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Abstract
Lyophilized and heat-treated cells from the 7 serotypes of S. mutans were examined for their ability to bind added insoluble-product glucosyltransferase (GTase) and to synthesize cell-associated glucan from [14C]sucrose. Lyophilized cells of serotypes a and g did not synthesize any more additional glucan than did the controls after exposure to GTase. These cells, however, synthesized 4- to 8-fold greater quantities of glucan than did the cells of the remaining serotypes. Lyophilized cells of serotypes b, c, d, e and f synthesized 2- to 3-fold greater quantities of glucan after exposure to GTase than did the controls without added enzyme. Lyophilized cells of serotypes a and g synthesized 6- to 10-fold greater quantities of glucan than did heat-treated cells of the same strain after binding of GTase. Lyophilized cells of the remaining serotypes synthesized only 1.6- to 3.3-fold-greater quantities of glucan than did the heat-treated cells. Heat treatment to inactivate cell-associated GTase does not create additional GTase binding sites in S. mutans and serotypes a and g are considerably more active in cell-associated glucan synthesis than cells of the other 5 serotypes. Ten species of gram-positive and gram-negative bacteria from 5 genera which do not produce in vitro plaque synthesized 10- to 100-fold-less glucan than did the S. mutans strains after exposure to GTase. Of these species, S. sanguis, Actinomyces viscosus and A. naeslundii synthesized the largest quantities of glucan. Three mutant strains of S. mutans which possess a reduced ability for in vitro adherence but do agglutinate with glucan or dextran synthesized only 1/3 as much glucan after binding of GTase as the control. These results were discussed in relation to in vitro and in vivo plaque development and the agglutination of S. mutans. The results support earlier findings which indicate that the presence of bacterial species other than S. mutans in smooth-surface dental plaque is due in part to contact of the cells with glucan in the developing plaque and not to the binding of cell-free GTase and the in situ synthesis of glucan. The results obtained with these representative strains of the 7 serotypes of S. mutans may not apply to the same extent to other strains within the serotypes.