Qualitative Changes of the Kunitz Trypsin Inhibitor in Soybean Seeds during Germination as Detected by Electrophoresis

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Abstract
Changes in the 3 forms of the Kunitz trypsin inhibitor protein (SBTI-A2) in 6 populations of soybeans [Glycine max] during germination were detected using polyacrylamide gel electrophoresis. In each population there was a shift in the mobility of the SBTI-A2 band(s) that appeared in the mature dry seed. The changes in the electrophoretic mobilities of the band(s) began about day 4 into germination and was completed by about day 6. Only part of the SBTI-A2 band(s) found in mature dry seed was changed to new forms during germination. There were no differences in the Rf [mobility relative to the front] distances between bands of the 3 types of SBTI-A2 found in mature seed and the Rf distances between the original and new bands found during germination. The new forms of SBTI-A2 noted during germination could be due to a modification of the original SBTI-A2 protein(s) by a protease.