Low molecular weight retinol- and retinoic acid-binding proteins were present in the soluble fraction of human retinal tissue but absent from human fibroblasts grown in tissue culture. By gel filtration and comparison with bovine retinal tissue, the human intracellular binding proteins had MW of approximately 17,000 daltons, comparable to the molecular weights of bovine intracellular binding proteins. The quantity of retinoic acid bound exceeded that of retinol by about 8-fold.