The membrane‐anchoring systems of vertebrate acetylcholinesterase and variant surface glycoproteins of African trypanosomes share a common antigenic determinant
- 21 April 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 199 (2) , 182-186
- https://doi.org/10.1016/0014-5793(86)80476-8
Abstract
Amphiphilic detergent-soluble acetylcholinesterase (AChE) from Torpedo is converted to a hydrophilic form by digestion with phospholipase C from Trypanosoma brucei or from Bacillus cereus. This lipase digestion uncovers an immunological determinant which crossreacts with a complex carbohydrate structure present in the hydrophilic form of all variant surface glycoproteins (VSG) of T. brucei. This crossreacting determinant is also detected in human erythrocyte AChE after digestion with T. brucei lipase. From these results we conclude that the glycophospholipid anchors of protozoan VSG and of AChE of the two vertebrates share common structural features, suggesting that this novel type of membrane anchor has been conserved during evolution.Keywords
This publication has 21 references indexed in Scilit:
- Identification of covalently attached fatty acids in the hydrophobic membrane-binding domain of human erythrocyte acetylcholinesteraseBiochemical and Biophysical Research Communications, 1985
- Phosphatidylinositol is the membrane-anchoring domain of the Thy-1 glycoproteinNature, 1985
- Solubilization of Membrane‐Bound Acetyicholinesterase by a Phosphatidylinositol‐Specific Phospholipase CJournal of Neurochemistry, 1985
- Novel Protein/Membrane AttachmentScience, 1985
- Synthesis of a hydrolase for the membrane‐form variant surface glycoprotein is repressed during transformation of Trypanosoma bruceiFEBS Letters, 1985
- Identification of covalently bound inositol in the hydrophobic membrane-anchoring domain of Torpedo acetylcholinesteraseBiochemical and Biophysical Research Communications, 1985
- Amphiphilic Detergent‐Soluble Acetylcholinesterase from Torpedo marmorata: Characterization and Conversion by Proteolysis to a Hydrophilic FormJournal of Neurochemistry, 1985
- Hydrophobic labeling of the membrane binding domain of acetylcholinesterase from Torpedo marmorataFEBS Letters, 1984
- The membrane form of variant surface glycoproteins of Trypanosoma bruceiNature, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970