μ‐type calcium‐activated neutral protease in the rat peripheral nerve

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Abstract
Previously we reported results of an incubation experiment with neurofilaments that supported the existence of a μ‐type of Ca2+‐activated neutral protease in the rat peripheral nerve; it was active with μM order Ca2+ (μ‐CANP). This time, we partially purified the μ‐CANP from a crude CANP fraction of rat peripheral nerve by using a DE52 column and a Phenyl‐Sepharose column followed again by DE52 column chromatography. The presence of μ‐CANP was verified by an immunoblotting technique. The μ‐CANP degraded the neurofilament triplet as previously reported; i.e., among the neurofilament triplet, the 160K component was most sensitive, and in the order of the 160K, 68K, and 200K components, respectively.