Comparison of antibiotic resistant and sensitive strains ofHaemophilus influenzae type b in The Netherlands by outer-membrane protein subtyping

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Abstract
The percentage ofβ-lactamase producingHaemophilus influenzae strains from patients with meningitis in The Netherlands increased from 0% in 1975/1976 to 4.6 % in 1985/1986 (n=1559). Twenty-three isolates resistant to ampicillin, penicillin, chloramphenicol, rifampicin and/or tetracycline were subtyped to determine if one resistant strain was spreading. (Sub)typing was performed by capsular typing, analysis of the major outer membrane protein patterns on sodium dodecylsulfate gels (SDS-PAGE subtypes), lipopolysaccharide serotyping and biotyping. The (sub)types of the resistant strains were similar to those of sensitive strains, thus indicating that antibiotic resistant strains develop at random.