Nesslerization Technic and its Effect on Serum Protein Values

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Abstract
Variations in nesslerizing technic produced marked alterations in the total serum protein values as detd. by the Campbell-Hanna method. Slow nesslerization down the side of the digestion tube caused a significant elevation of the serum protein levels as compared to total protein values secured on the same samples by other methods. To obtain reproducible total serum protein values as well as values comparable to those obtained on the same samples by other methods, Nessler''s reagent must be added rapidly into the center of the soln. in the digestion tube, and the resulting soln. immediately mixed by inversion. Other sources of error in the Campbell-Hanna method such as contamination of the acid digestion mixture and the superoxol with nitrogenous compounds, and contamination of the samples during digestion due to the presence of nitrogenous gases in the hood, were investigated by the author and shown to be insignificant.

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