Properties of ATP-dependent H+-transport in rat brain synaptosomes.
- 1 January 1986
- journal article
- research article
- Published by Japanese Pharmacological Society in Folia Pharmacologica Japonica
- Vol. 88 (6) , 443-448
- https://doi.org/10.1254/fpj.88.443
Abstract
ラット脳の神経終末形質膜におけるATP依存性のH+輸送をacridine orangeの蛍光減弱により検索した．ATP添加後の膜顆粒内H+蓄積を示す蛍光減弱は，低張処理した神経終末では観察されたが，無処理の神経終末では観察されなかった．低張処理した神経終末のH+輸送活性は反応液のアニオンがCl-かBr-の場合にのみ観察され，Mes-，NO3-，I-およびSCN-では消失した．反応液のK+をCs+，Na+またはLi+に置き換えると，K+>Gs+>Na+>Li+の順にH+輸送活性は減少した．このH+輸送活性は，0.3 mM ethacrynic acidでは対照の39.2%に，10μM 4-acetamide-4′-isothiocyanatostilbene-2,2′-disulfonic acidでは36.3%に，また1 mM 4-aminopyridineでは33.1%にまで阻害されたが，1 mM ouabain，500 μM vanadate， 10 μM picrotoxinおよび100 μMγ-aminobutyric acidでは阻害されなかった．これらの結果から，神経終末において，ATP依存性H+輸送系のATP作用部位は細胞内側表面に存在し，Cl-またはBr-の存在，およびK+のK+チャンネルを介する移動が，H+輸送活性を高めることが示唆された．This publication has 15 references indexed in Scilit:
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