Integrated planar terahertz resonators for femtomolar sensitivity label-free detection of DNA hybridization
- 1 April 2002
- journal article
- Published by Optica Publishing Group in Applied Optics
- Vol. 41 (10) , 2074-2078
- https://doi.org/10.1364/ao.41.002074
Abstract
A promising label-free approach for the analysis of genetic material by means of detecting the hybridization of polynucleotides with electromagnetic waves at terahertz (THz) frequencies is presented. Using an integrated waveguide approach, incorporating resonant THz structures as sample carriers and transducers for the analysis of the DNA molecules, we achieve a sensitivity down to femtomolar levels. The approach is demonstrated with time-domain ultrafast techniques based on femtosecond laser pulses for generating and electro-optically detecting broadband THz signals, although the principle can certainly be transferred to other THz technologies.Keywords
This publication has 27 references indexed in Scilit:
- Sensitive detection method of dielectric dispersions in aqueous-based, surface-bound macromolecular structures using microwave spectroscopyApplied Physics Letters, 1999
- Parallel analysis of genetic selections using whole genome oligonucleotide arraysProceedings of the National Academy of Sciences, 1998
- Comparison of fluorescein isothiocyanate- and Texas red-conjugated nucleotides for direct labeling in comparative genomic hybridizationCytometry, 1998
- Molecular mechanism controlling the incorporation of fluorescent nucleotides into DNA by PCRCytometry, 1997
- Accessing Genetic Information with High-Density DNA ArraysScience, 1996
- Real-time detection and quantification of DNA hybridization by an optical biosensorAnalytical Chemistry, 1995
- Directly labeled DNA probes using fluorescent nucleotides with different length linkersNucleic Acids Research, 1994
- DNA Sequence Determination by Hybridization: a Strategy for Efficient Large-Scale SequencingScience, 1993
- The estimation of distances between specific backbone-labeled sites in DNA using fluorescence resonance energy transferNucleic Acids Research, 1992
- Resonant Microwave Absorption of Selected DNA MoleculesPhysical Review Letters, 1984