Inhibition of DNAse activity in PFGE analysis of DNA from Campylobacter jejuni
Open Access
- 1 November 1994
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 19 (5) , 357-358
- https://doi.org/10.1111/j.1472-765x.1994.tb00474.x
Abstract
DNAse-positive strains of Campylobacter jejuni degrade their chromosomal DNA during standard preparative procedures before pulsed-field gel electrophoresis (PFGE). A simple method for inactivation of this DNAse activity is described. Formaldehyde fixation of the bacterial cells resulted in the preservation of the DNA in a state suitable for restriction digestion and subsequent electrophoretic analysis.Keywords
This publication has 9 references indexed in Scilit:
- Typing of group B streptococci: comparison of pulsed-field gel electrophoresis and conventional electrophoresisJournal of Clinical Microbiology, 1993
- Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unitJournal of Hospital Infection, 1991
- Resolution of Recent Evolutionary Divergence among Escherichia coli from Related Lineages: The Application of Pulsed Field Electrophoresis to Molecular EpidemiologyThe Journal of Infectious Diseases, 1990
- SAMPLE PREPARATION FROM PARAFFIN-EMBEDDED TISSUESPublished by Elsevier ,1990
- In situ hybridizationMolecular and Cellular Probes, 1987
- New, extended biotyping scheme for Campylobacter jejuni, Campylobacter coli, and "Campylobacter laridis"Journal of Clinical Microbiology, 1984
- Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry.Journal of Histochemistry & Cytochemistry, 1983