Comparison of Various Tissue Cultures for the Isolation of Enteroviruses

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Abstract
The number of enteroviruses isolated from sewage samples in parallel tests in 4 kinds of tissue cultures was used to establish the relative sensitivities of the cultures to the agents. Monkey kidney tissue cultures were more sensitive to polioviruses and to ECHO viruses Types 1, 6, and 8 than were the other kinds of cultures; HeLa cell cultures were the most sensitive to Coxsackie virus Group B Type 5, and amnion tissue cultures were the most sensitive to the other ECHO viruses. The use of both monkey kidney and human amnion tissue cultures is recommended for the isolation of enteroviruses.