Stimulus specificity of the generation of leukotrienes by dog mastocytoma cells.

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Abstract
Isolated dog mastocytoma cells sensitized with dog anti-ragweed IgE and challenged with ragweed antigen or incubated with ionophore A23187 [calcimycin] or the carboxy-terminal dodecapeptide of platelet factor 4. PF4(59-70), release histamine and concurrently generate leukotrienes B4, C4 and D4. The exposure of mastocytoma cells to 0.1-3 .mu.g/ml of 15-hydroxyeicosatetraenoic acid stimulates selectively the generation of leukotrienes, in the absence of histamine release, while 0.1-1 .mu.g/ml of compound 48/80 releases histamine without enhancing the generation of leukotrienes. That natural stimuli are capable of selectively activating 1 synthetic or secretory compartment of mast cells suggests that separate subsets of receptors as well as different biochemical events may serve to mobilize each class of mediators.