Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mers
- 15 February 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 228 (2) , 228-230
- https://doi.org/10.1016/0014-5793(88)80004-8
Abstract
Two fragments (21‐mers) containing consensus sequences for the self‐cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3′‐terminal 2′,3′‐cyclic phosphate. Twenty‐five combinations of partially double‐stranded 21‐mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self‐cleavage. It seemed that guanosine 3′‐phosphate was not susceptible to transesterification at the cleavage site.Keywords
This publication has 11 references indexed in Scilit:
- A small catalytic oligoribonucleotideNature, 1987
- Self-cleavage of virusoid RNA is performed by the proposed 55-nucleotide active siteCell, 1987
- Self-cleavage of plus and minus RNAs of a virusoid and a structural model for the active sitesCell, 1987
- Self-cleaving transcripts of satellite DNA from the newtCell, 1987
- Satellite tobacco ringspot virus RNA: A subset of the RNA sequence is sufficient for autolytic processingProceedings of the National Academy of Sciences, 1986
- Non-enzymatic cleavage and ligation of RNAs complementary to a plant virus satellite RNANature, 1986
- Autolytic Processing of Dimeric Plant Virus Satellite RNAScience, 1986
- Self-cleavage of plus and minus RNA transcripts of avocado sunblotch viroidNucleic Acids Research, 1986
- DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mappingNucleic Acids Research, 1974
- Use of DNA Polymerase I Primed by a Synthetic Oligonucleotide to Determine a Nucleotide Sequence in Phage f1 DNAProceedings of the National Academy of Sciences, 1973