Relative incorporation of acetate and glucose into glycerides ofGlomerella cingulata

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Abstract
Cultures of the fungusGlomerella cingulata were pulse-labeled for 30 min with 1-14C-acetate at ages from 2 to 12 days old. The greatest incorporation into triglycerides and phosphoglycerides occurred in the youngest cultures, but more of the radioactive acetate was diverted to the phosphoglycerides than to the triglycerides in these cultures. In another series of experiments the incorporation of 1-14C-acetate or 2-14C-glucose into the triglycerides and phosphoglycerides of the fungus was investigated at 15-minute intervals for 1 hr. Hydrolysis of the two classes of glycerides revealed some labeling of glycerol from acetate; but, not surprisingly, acetate was incorporated into the fatty acid moieties to a greater extent than into the glycerol moieties, and there was relatively greater incorporation of 2-14C-glucose into glycerol than into fatty acids. Some relationships of these results to the growth and development ofG. cingulata are suggested, and implications relative to control mechanisms are pointed out.