A STANDARDIZED BIOASSAY FOR LARVICIDAL ACTIVITY OF BACILLUS THURINGIENSIS IN SUNFLOWER MOTH HOMOEOSOMA ELECTELLUM (LEPIDOPTERA: PHYCITIDAE)
- 1 January 1985
- journal article
- research article
- Published by Cambridge University Press (CUP) in The Canadian Entomologist
- Vol. 117 (1) , 15-22
- https://doi.org/10.4039/ent11715-1
Abstract
A standard test for the larvicidal activity of Bacillus thuringiensis (B.t.) against the larvae of the sunflower moth Homoeosoma electellum (Hulst) has been developed. Bioassay parameters investigated include diet preparation, concentration of B.t., effect of formaldehyde, and method of pathogen incorporation in diet. The LC50 for seconded third-instar larvae is 1.24 μg of Dipel® WP ml−1 or 19.8 IU B.t. ml−1 pathogen-incorporated diet. Layering of a pathogen-suspension upon the surface of the diet was not a reliable bioassay technique for H. electellum. The addition of formaldehyde in the diet reduced the slope of the dose–mortality curve but did not change the LC50. Un test standard d'activité larvicide de Bacillus thuringiensis (B.t.) contre les larves de pyrale de tournesol, Homoeosoma electellum (Hulst) a été développé. Les paramètres suivants de bioassay on été exploré : préparation de l'aliment; concentration de B.t.; effet de la formaldehyde; le procédé pour incorporer le pathogène dans l'aliment. Le LC50 pour les larves du deuxième et troisième instar est 1.24 μg/ml Dipel® ou 19.8 IU/ml B.t. pathogène incorporé dans l'aliment. Pour la technique de bioassay, une couche de nés pension pathogène sur la surface de l'aliment ne s'est pas avère satisfaisant. Dans l'aliment, l'addition de la formaldehyde a réduit la pente de la courbe de dose-mortalité, mais n'a pas changé le LC50.This publication has 11 references indexed in Scilit:
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