An efficient solid-phase strategy for the construction of chemokines
- 13 October 2000
- journal article
- research article
- Published by Wiley in Journal of Peptide Science
- Vol. 6 (10) , 512-518
- https://doi.org/10.1002/1099-1387(200010)6:10<512::aid-psc269>3.0.co;2-a
Abstract
Synthesis of chemokines via stepwise SPPS approaches has been shown to be a challenge. Herein, a complete study of different coupling methods, solvents and temperature combined with a continuous-flow synthesizer equipped with feedback monitoring was carried out. The results from this study indicate that this family of molecules can be prepared using an Fmoc/But chemical approach and provide a general method to apply for the elongation of other difficult sequences. Copyright © 2000 European Peptide Society and John Wiley & Sons, Ltd.Keywords
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