Release of 7-methylguanine residues from alkylated DNA by extracts of Micrococcus luteus and Escherichia coli.
- 1 February 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (2) , 852-855
- https://doi.org/10.1073/pnas.78.2.852
Abstract
Cell extracts from M. luteus release both free 3-methyladenine and free 7-methylguanine from alkylated [calf thymus] DNA. The glycosylase activity responsible for the liberation of 7-methylguanine is not 3-methyladenine-DNA glycosylase, which, when purified, does not liberate it. The heat inactivation rates of the 2 enzymatic activities are different. The release of 7-methylguanine by chemical depurination of ethanol-soluble oligonucleotides was ruled out. A similar activity releasing 7-methylguanine is also found in E. coli.This publication has 27 references indexed in Scilit:
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