A 6 bp Z‐DNA hairpin binds two Zα domains from the human RNA editing enzyme ADAR1
Open Access
- 3 September 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 458 (1) , 27-31
- https://doi.org/10.1016/s0014-5793(99)01119-9
Abstract
The Zα domain of the human RNA editing enzyme double‐stranded RNA deaminase I (ADAR1) binds to left‐handed Z‐DNA with high affinity. We found by analytical ultracentrifugation and CD spectroscopy that two Zα domains bind to one d(CG)3T4(CG)3 hairpin which contains a stem of six base pairs in the Z‐DNA conformation. Both wild‐type Zα and a C125S mutant show a mean dissociation constant of 30 nM as measured by surface plasmon resonance and analytical ultracentrifugation. Our data suggest that short (≥6 bp) segments of Z‐DNA within a gene are able to recruit two ADAR1 enzymes to that particular site.Keywords
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