Abstract
1. A number of varieties of winter wheat, known to vary considerably in degree of winter hardiness, were compared in the hardened condition with reference to the physical constants of the cell sap, and the content of dry matter, nitrogen, sugars and starch. 2. No constant relation was found between depression of the freezing point, specific conductivity, or hydrogen-ion concentration of the cell sap and relative frost hardiness. 3. Sugars accounted for 34 to 38 per cent, of the total osmotic pressure of the sap.