Golgi Method without Osmium Tetroxide for the Study of the Central Nervous System
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Biotechnic & Histochemistry
- Vol. 67 (5) , 288-296
- https://doi.org/10.3109/10520299209110037
Abstract
A variant Golgi technique was developed that consisted of substituting osmium tetroxide with formaldehyde as the initial fixative in intracardiac perfusion, along with the addition of glacial acetic acid to the chromating fluid. This procedure avoids disposal of dangerous waste substances into the environment. Other advantages include 1) reduction of cost, danger to lab workers, and risk of disruption of the tissue slices during their handling by eliminating the osmium tetroxide, 2) clear tissue background, 3) greater quantity of impregnated neurons than in the classical procedure, with distinct morphological details easily identified even in gross sections and 4) reduction in processing time.Keywords
This publication has 13 references indexed in Scilit:
- Synaptic Relationships of Golgi-Impregnated Neurons as Identified by Electrophysiological or Immunocytochemical TechniquesPublished by Springer Nature ,1989
- Chromation at low temperatures improves impregnation of neurons in Golgi-aldehyde methodsJournal of Neuroscience Methods, 1986
- The Golgi MethodsPublished by Springer Nature ,1981
- A new procedure for examining Golgi impregnated neurons by light and electron microscopyJournal of Neurocytology, 1977
- Staining Formalin-Fixed Nerve Tissue with Mercuric NitrateStain Technology, 1959
- Improvement of the Golgi Method by pH ControlStain Technology, 1957
- Golgi'S Dichromate-Silver Method 3. Chromating FluidsStain Technology, 1954
- Zinc Chromate Modification of the Golgi TechnicStain Technology, 1951
- The Use of Formalin in NeurologyTransactions of the American Microscopical Society, 1896
- Imprägnation des centralen Nervensystems mit QuecksilbersalzenArchiv für Mikroskopische Anatomie, 1891