El cultivo "in vitro" como instrumento práctico para el diagnóstico y aislamiento primario de Leishmania braziliensis braziliensis. 2. Estudios en pacientes de áreas endémicas
Open Access
- 1 October 1986
- journal article
- research article
- Published by FapUNIFESP (SciELO) in Revista do Instituto de Medicina Tropical de São Paulo
- Vol. 28 (5) , 317-324
- https://doi.org/10.1590/s0036-46651986000500006
Abstract
In vitro cultivation of Lbb is a useful method in the field for primary isolation of this leismanial subspecies. Cultures in biphasic blood agar media (DAB and Evans) and liquid media (Schneiders and AR 103) done in patients with cutaneous leismanias showed. A similar sensibility in DAB and Schneiders medium when needle aspirates were used to seed the cultures; That DAB medium performed well when compared with Evans medium; A rise in isolation of Lbb if 3 aspirations taken from different sites in the same lesion were cultivated (50% postivity with DAB medium).Keywords
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