DETECTION OF HUMAN OSTEOSARCOMA-ASSOCIATED ANTIGEN(S) BY MONOCLONAL-ANTIBODIES

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (2) , 654-659
Abstract
Hybrid cell lines were derived from a fusion between mouse myeloma cells, NS1, and spleen cells from mice immunized with freshly resected osteosarcoma cells from an untreated patient. Of the 276 hybrids obtained, 5 secreted antibodies which bound to osteosarcoma tissues but not to autologous skin fibroblasts. The antibodies from 3 of these 5 hybrids, OST6, OST7 and OST15, reacted with all of 5 osteosarcoma tissues and with 1 chondrosarcoma tissue but not with other malignant or benign tumors. Tests of various normal tissues were negative, except for weak binding to a subpopulation of chondrocytes in articular cartilage. The reciprocal binding inhibition test showed that OST6, OST7 and OST15 antibodies were directed against different antigenic determinants.