IMMUNOCHEMICAL VISUALIZATION OF BINDING OF THE CHEMICAL CARCINOGEN BENZO(A)PYRENE DIOL-EPOXIDE 1 TO THE GENOME
- 1 January 1981
- journal article
- research article
- Vol. 41 (8) , 3111-3117
Abstract
Antisera against DNA modified with r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo(a)pyrene (BPDE-1) was elicited in rabbits. Such sera reacted with single- or double-stranded modified DNA but not with unmodified DNA, free benzo(a)pyrene or proteins modified by BPDE-1. Indirect immunofluorescence studies indicated that the IgG in the sera bound specifically to the nuclei of KD cells which were treated with BPDE-1. The intensity of fluorescence was proportional to the dose of BPDE-1 used to treat the cells. About 50% of the BPDE-1-DNA adducts remained bound to DNA 24 h after the removal of the carcinogen. The location of BPDE-1-modified bases in Col E1 DNA was visualized by immunoelectron microscopy.This publication has 16 references indexed in Scilit:
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