Genetic identification of cloned fragments of bacteriophage T4 DNA and complementation by some clones containing early T4 genes
- 1 January 1977
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 154 (3) , 319-326
- https://doi.org/10.1007/bf00571289
Abstract
Bacteriophage T4 DNA containing cytosine has been obtained from cells infected with phage mutant in genes 42, 56,denA anddenB. This DNA can be cut by a number of restriction endonucleases. Fragments obtained by digestion of this DNA withEcoRI have been cloned using the vector plasmid pCR1. Clones containing T4 DNA were identified by hybridization with radioactive early and late T4 RNA. A simple marker rescue technique is described for the genetic identification of the cloned T4 fragments. Some of the T4-hybrid plasmids which contain entire T4 genes can complement temperature sensitive and amber mutants of T4.Keywords
This publication has 21 references indexed in Scilit:
- T4 Bacteriophage-coded RNA polymerase subunit blocks host transcription and unfolds the host chromosomeNature, 1977
- In vitro construction of bacteriophage λ and plasmid DNA molecules containing DNA fragments from bacteriophage T4Gene, 1976
- The production of undegraded cytosine-containing DNA by bacteriophage T4 in the absence of dCTPase and endonucleases II and IV, and its effects on T4-directed protein synthesisJournal of Molecular Biology, 1975
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975
- Characterization of a new class of deletions of the D region of the bacteriophage T4 genomeVirology, 1975
- A system for mapping DNA sequences in the chromosomes of Drosophila melanogasterCell, 1974
- Regulation of the synthesis of bacteriophage T4 gene 32 proteinJournal of Molecular Biology, 1974
- Studies with glucosyl transferase mutants of the T-even bacteriophagesVirology, 1971
- Control of synthesis of glucosyl transferase and lysozyme messengers after T4 infectionJournal of Molecular Biology, 1970
- Studies on the nature of replicating DNA in T4-infected Escherichia coliJournal of Molecular Biology, 1966