Characterization of N-Glycolylneuraminic Acid-Containing Glycosphingolipids from a Marek's Disease Lymphoma-Derived Chicken Cell Line, MSBI, as Tumor-Associated Heterophile Hanganutziu-Deicher Antigens1
- 1 March 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 95 (3) , 785-794
- https://doi.org/10.1093/oxfordjournals.jbchem.a134670
Abstract
Heterophile, Hanganutziu-Deicher (HD) antigen-active N -glycolylneuraminic acid-containing glycosphingolipids (GSLs) were detected as tumor-associated foreign antigens of a Marek's disease lymphoma-derived cell line, MSB1, by enzyme-immunoassay with chicken antibody against N -glycolylneuraminyl-lactosylceramide (anti-NeuGc-LacCer). At least three species of HD antigen-active GSLs were detected by two-dimensional thin-layer chromatography (TLC) combined with enzyme-immuno-assay. The reactivities of the GSLs with anti-NeuGc-LacCer, their behaviors on two-dimensional TLC and the results of an endo-β-galactosidase digestion study indicated that these three GSLs were NeuGc-LacCer (NeuGcα2–3Galβ1-4Glc-Cer), NeuGc-nLcOse 4 Cer (NeuGcα2–3Galβ1-4GIcNAcβ1–3Galβ1–4Glc-Cer) and NeuGc-nLcOse 6 Cer (NeuGcα2–3Galβ1–4GlcNAcβ1–3Galβ1–4GlcNAcβ1–3Galβ1–4Glc-Cer).Keywords
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